Microbial Identification System

Biolog’s powerful carbon source utilization technology accurately identifies environmental and pathogenic microorganisms by producing a characteristic pattern or “metabolic fingerprint” from discrete test reactions performed within a 96 well microplate.

Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to extensive databases of environmental organisms, human pathogens, veterinary pathogens and plant pathogens. The scope of the 96 assay reactions, coupled with sophisticated interpretation software, delivers a high level of accuracy that is comparable to molecular methods. The one minute per sample set up is much simpler and faster than DNA sequencing and the automated pattern matching eliminates the need for training and expertise in gene sequence interpretation. Provides all the required functionality to support 21CFR Part 11 electronic record requirements and regulatory compliance.

Biolog’s latest generation redox chemistry enables testing and identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed. A simple, one minute setup protocol is used for each sample. The expanded GEN III database is designed to meet the needs of Biolog’s broad customer base covering diverse disciplines of microbiology.

  • • NO Gram stain
  • • NO pre-tests
  • • NO follow-on tests
  • • ONE panel for both GN & GP bacteria
  • • ONE minute set-up
  • • OVER 2900 species coverage

All Biolog Microbial Identification Systems — manual, semi-automated or fully automated — use the powerful GENIII MicroPlate™. The GEN III redox chemistry is applicable to an unprecedented range of both Gram-negative and Gram-positive bacteria. GENIII dissects and analyzes the ability of the cell to metabolize all major classes of biochemicals, in addition to determining other important physiological properties such as pH, salt, and lactic acid tolerance, reducing power, and chemical sensitivity.

Omnilog® ID System:

  • Fully automated high throughput aerobic identification
  • 96 well MicroPlates incorporate a patented Redox tetrazolium dye that changes color as a result of cellular respiration providing a metabolic fingerprint.
  • Offers a simple automated process to meet the dynamic workflow demands of a wide range of microbiology applications.
  • Even organisms requiring special incubation temperatures or environments are easily accommodated, ensuring accurate identification for a broad spectrum of Gram-negative and Gram-positive bacteria.
  • Fully automates the process of microbial identification by incubating, reading and interpreting results from up to 50 Biolog MicroPlates™ at a time.
  • The system software provides directives to guide all steps of the testing process for each MicroPlate, including sample placement and removal and extended incubation when needed.
  • Additional MicroPlates can be loaded and processed any time there is an open tray or identification has been completed on a previously entered MicroPlate.
  • Up to date information for each MicroPlate, including results as they become available, are continuously visible on the system’s menu screen.
  • Identifications take as little as 2 hours.

MicroStation™ ID System:

  • Semi-automated identification for bacteria, yeast, and filamentous fungi
  • 96 well MicroPlates incorporate a patented Redox tetrazolium dye that changes color as a result of cellular respiration providing a metabolic fingerprint.
  • Versatile system, with the ability to identify and characterize a wide range of environmental and pathogenic organisms across diverse fields of microbiology.
  • Using all Biolog databases, over 2650 species of bacteria, yeast and filamentous fungi can be identified.
  • Over 2900 species of bacteria, yeast and filamentous fungi can be identified
  • Some bacteria can be identified in as little as 2 hours.
  • Just prepare a cell suspension and inoculate the appropriate MicroPlate. After inoculation and incubation, the MicroPlate is placed into the MicroStation Reader for analysis. The unique metabolic pattern generated by the organism is recorded and compared to hundreds of identification profiles in a corresponding Biolog Database.
  • The versatile plate reader uses dual wavelength readings to quantify color reactions in the MicroPlate wells, adding consistency and accuracy when reading the reaction patterns.
  • Biolog’s patented redox chemistry makes use of different carbon compounds including sugars, carboxylic acids, amino acids and peptides to provide an unparalleled wealth of discriminating biochemical characterizations.
  • This diverse set of tests enables these systems to identify microorganisms that other kit-based methods misidentify or fail to identify.

    Another option, The OmniLog® Combo System, provides the microbiologist with a unique solution for both microbial identification and characterization of cellular functions in a single system.

    From routine species level identification to projects in detailed strain characterization, such as finger printing and tracking strains that cause product contamination, comparison of non-pathogenic and pathogenic strains, gene and mutant characterization, cell line quality control, and bioprocess improvement.
    Researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, and exposure to drugs and other chemicals. They can correlate genotypes with phenotypes, determine a cell’s metabolic and chemical sensitivity properties, discover new targets for antimicrobial compounds, optimize cell lines and culture conditions in bioprocess development, characterize cell phenotypes for taxonomic or epidemiological studies, and more.

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